Sartorius, 5ZALB-0002, HEA HyperCel

HEA HyperCel is a Mixed Mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH.

HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “Mixed Mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).
Features and Benefits
High mechanical and chemical stability
Suitable for large-scale chromatography in a biopharmaceutical environment
Cross-linked cellulose base matrix
Applications
Protein purification at low ionic strength by direct hydrophobic capture
Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
Orthogonal separation mechanism to ion exchange or HIC

Specifications
Compliance Information:
Quality Standards: CoA; RSF

Dimensions:
Bead Size: 80 - 100 µm

Materials of Construction:
Base Matrix: Rigid cellulose beads

Operating Conditions:
Operating Pressure: < 3 barg (44psi)
pH Stability: 1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information:
Ligand: Hexylamine
Reference Protein: Bovine serum albumin (BSA)
Typical Dynamic Binding Capacity 10% per mL: 40-60 mg/mL BSA

Product Information:
Brand: HEA HyperCel
Resin Type: Mixed Mode anion exchanger

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl