Sartorius, 611.5118-2, CIMmultus PrimaS® 80 mL Monolithic Column (2 μm)

This multimodal column combines anion exchange and hydrogen bonding properties, making its selectivity entirely distinct from traditional anion exchangers.
It is most often employed for the capture of RNA in crude samples such as IVT mixtures.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
The ligand in PrimaS® contains a series of hydrogen donors and hydrogen acceptors, combined with a weak anion exchange character. Its hydrogen bonding ability makes its selectivity entirely distinct from traditional anion exchangers. PrimaS purifies large single-stranded mRNA (ssRNA) under aqueous conditions at ambient temperature. Contaminants are removed using a high salt wash, while ssRNA is recovered from the column with a pH gradient. It can be used for purification of ssRNA, including RNA exceeding 10 kb (such as self-amplifying RNA). In a purification process, it is most often employed for the capture of RNA in crude samples such as IVT mixtures.
PrimaS® features:
Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
Offers unique selectivities in many important product areas
Supports purification of single-stranded RNA at ambient temperature
Fractionates ssRNA by size
Your benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: non-cGMP

Connections (Physical):
Connector(s): TC 1" (25 mm), 3 mm ID bore

Dimensions:
Channel Size: 2 µm
Column Tube Length: 110 mm
Inner Diameter: 15 mm
Outer Diameter: 34 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 8–400 mL/min | 6–300 cm/h | 0.1–5 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 2.0 MPa | 20 bar | 290 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 2–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 80 mL
Ligand: PrimaS (multimodal - anion exchange-hydrogen bonding)

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 80 mL