Sartorius, 911.6157-2, CIMmultus® SO3 80 mL cGMP Compliant Monolithic Column (2 μm)

This high-performance cation exchange column is primarily intended for fast and efficient purification of biomolecules such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus® can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CIMmultus® SO3 is a high-performance cation exchange column, primarily intended for fast and efficient purification of biomolecules, such as viruses, adeno-associated viruses (AAV), and virus-like particles (VLP) through their ionic interaction with the monolith.
SO3 (sulfonate) features:
Strong cation exchanger
Working range, pH 2–13
Binds molecules with predominantly positive charge, repels molecules a predominantly negative charge
Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
Main application: AAV capture step
High capacity for purification of virus and VLP particles, large proteins
Sanitizable with 1 M NaOH
Benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: cGMP

Connections (Physical):
Connector(s): TC 1" (25 mm), 3 mm ID bore

Dimensions:
Channel Size: 2 µm
Column Tube Length: 110 mm
Inner Diameter: 15 mm
Outer Diameter: 34 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 8–400 mL/min | 6–300 cm/h | 0.1–5 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 2.0 MPa | 20 bar | 290 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 2–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 80 mL
Ligand: SO3 (strong cation exchanger, sulfonate)
Typical Dynamic Binding Capacity 10% per mL: ≥ 21 mg Lysozyme/mL wet support, Lysozyme 3.0 mg/mL, 20 mM Tris-HCl buffer, pH 8.0, flow rate 160 mL/min

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 80 mL