Sartorius, 931.8136-2, CIMmultus® C4 HLD 8000 mL cGMP Compliant Monolithic Column (2 μm)

CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.

CIMmultus® is a unique and industry-proven line of pre-packed chromatographic monolithic columns featuring large flow-through channels, designed for working with large biomolecules such as viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), nucleic acids (mRNA, pDNA), virus-like particles and exosomes. CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes to 8000mL for industrial manufacturing.
CIMmultus® C4 HLD employs a high density of butyl ligands to achieve strong surface hydrophobicity. Relying on hydrophobic interaction chromatography (HIC), this column is a must for nucleic acid separations, providing an excellent solution for the purification of pDNA and RNA.
C4 (butyl) high ligand density (HLD) features:
Strongly hydrophobic, uncharged
Working range, pH 2–10
Highly effective for removing proteins from nucleic acids (pDNA, RNA)
Your benefits:
Very fast processes by convective mass transport
Resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: cGMP

Connections (Physical):
Connector(s): TC 1" (25 mm), 8 mm ID bore

Dimensions:
Channel Size: 2 µm
Column Tube Length: 410 mm
Inner Diameter: 255 mm
Outer Diameter: 300 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 800–8000 mL/min | 13–135 cm/h | 0.1–1 CV/min

Materials of Construction:
Materials of Construction: Poly(butyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 0.7 MPa | 7 bar | 100 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 3–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 8000 mL
Ligand: C4 HLD (hydrophobic, butyl – high ligand density)

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 8000 mL