Sartorius, 97048, iQue® Human Multiplex Antibody Internalization Kit

The iQue® Multiplex Antibody Internalization Kits are high throughput, multiplexed, no-wash assays that measures antibody internalization, cell specificity, and cell health using 10 µL sample of cells and 10 µL antibody. Available in 1 x 96, 5 x 96, 1 x 384 and 5 x 384-well format.
Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.

Key Benefits
No wash protocol- fast, easy antibody-labeling method optimal for screening antibody internalization.
High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 µL of cells and 10 µL of labeled antibody (kit).
Simultaneously measure antibody internalization, cell specificity, and cell health within the same well.
Principle
Antibodies are easily labeled with Intellicyt Antibody Internalization Reagent, a novel pH sensitive dye. The labeled antibodies have little fluorescence at neutral pH, but become highly fluorogenic at low pH when they are internalized and processed through the acidic lysosome/endosome pathway.
Cell viability can also be measured to assess general cell health and antibody function. Using the same sample, cell specificity can be characterized using encoding dyes (cell populations/types). Data acquisition is performed using the iQue3 and ForeCyt® software for data analysis and visualization.

Specifications
Applications:
Application: Antibody Characterization
Research Area: Antibody Discovery

Biological Information:
Target: Human

General Specifications:
Laser Configuration: Violet-Blue-Red
Reagents and Kits Type: Multiplex Antibody Internalization (Human)

Product Information:
Brand: iQue®
Pack Size: 1 X 384 wells