Sartorius, BIA-1014.8000-2, CIMmultus® CDI 4000 mL Monolithic Column (2 μm)

CDI monolithic chromatographic columns are intended for the covalent immobilisation of sensitive molecules, such as peptides, proteins and oligonucleotides.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CDI monolithic chromatographic columns are used for covalent immobilisation of ligands (e.g. proteins, peptides and other amine or thiol containing molecules). The covalent nature of the bond between the ligand and matrix reduces leaching and improves stability and reusability. Immobilised supports enable a variety of customised analytical affinity chromatography options with interactions specific to the target molecule.
CDI (Carboxyimidazole) features:
Forms a covalent carbamate linkage with amino and thiol-containing molecules
Suitable for coupling of sensitive molecules, such as peptides, proteins, oligonucleotides
Benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: non-cGMP

Connections (Physical):
Connector(s): TC 1" (25 mm), 8 mm ID bore

Dimensions:
Channel Size: 2 µm
Column Tube Length: 205 mm
Inner Diameter: 255 mm
Outer Diameter: 300 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 400–4000 mL/min | 13–135 cm/h | 0.1–1 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 0.7 MPa | 7 bar | 100 psi
Recommended Operating Temperature: 4 °C (39 °F) to 35 °C (95 °F)
pH Stability: Working range 4–11

Physicochemical Information:
Bed Volume: 4000 mL
Ligand: CDI (activated; carboxy imidazole)

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 4000 mL