Sartorius, BIA-1014.8140-6, CIMmultus® OH 4000 mL Monolithic Column (6 µm)

This column relies on hydrophobic interaction chromatography (HIC) to provide an excellent solution for the purification of large biomolecules, such as virus particles, VLPs and extracellular vesicles.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.
CIMmultus® columns can be used as single-use disposable or multi-use columns. They are available in a variety of volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CIMmultus® OH relies on hydrophobic interaction chromatography (HIC) to provide an excellent solution for the purification of large biomolecules, such as virus particles, VLPs and extracellular vesicles.
OH (hydroxy) features:
Uncharged (neutral) ligand
Working range, pH 2–10
Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size
Can be used as a pre-capture step to reduce most host cell impurities in AAV purification
Elution with descending salt or polyethylene glycol
Sanitizable with 1 M NaOH
Benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: non-cGMP

Connections (Physical):
Connector(s): TC 1" (25 mm), 8 mm ID bore

Dimensions:
Channel Size: 6 µm
Column Tube Length: 205 mm
Inner Diameter: 255 mm
Outer Diameter: 300 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 400–12000 mL/min | 13–404 cm/h | 0.1–3 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 0.5 MPa | 5 bar | 70 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 3–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 4000 mL
Ligand: OH (hydrophobic, hydroxyl)

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 4000 mL