Thermo Fisher, 10533073, Terminal Deoxynucleotidyl Transferase, recombinant

Terminal Deoxynucleotidyl Transferase, recombinant Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.
Applications:Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2),32P- or35S-label (3), or in apoptosis (TUNEL) (4,5).
Source:Purified from E. coli clone of calf thymus TdT.
Performance and Quality Testing:Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition:One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50as a primer.
Hazard Warning:Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions:0.
2 M potassium cacodylate (pH 7.
2), 10 mM MgO4C4H6, 1 mM 2-mercaptoethanol, 0.
5 mg/ml BSA,100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.
15 ml for 1 h at 37°C.
Compatible Buffer: 5X Buffer, Reaction Buffer
Product Type: TdT
Quantity: 3 x 500 U
Enzyme: Terminal Deoxynucleotidyl Transferase
Unit Size: Each