Thermo Fisher, 10701D, Dynabeads™ Intact Virus Enrichment

10 mL Dynabeads Intact Virus Enrichmentbeads are 1-μm strong anion exchange magnetic beads that bind negatively charged vesicles and molecules. The beads can enrich for viruses such as, but not limited to, SARS-CoV-2 coronavirus, human immunodeficiency virus (HIV), respiratory syncytial virus (RSV), norovirus, adenovirus, enterovirus, and Ebola-, Zika-, Influenza A- viruses.
The enrichment is gentle and fast and can be performed in as little as 15 minutes. The virus can be easily removed from the beads in an additional 10-minute release step.
The Dynabeads Intact Virus Enrichment beads can also be used to isolate extracellular vesicles (EV’s) and exosomes with the same rapid and efficient enrichment and release method. Successful enrichment of exosomes has been performed from cell culture media and liquid biopsy samples such as serum, plasma and urine. In addition, the Dynabeads magnetic beads allow for high-throughput enrichment in combination with any of ourKingFisher instruments, thus both manual and automated protocols are available.
Features of the Dynabeads Intact Virus Enrichment beads include:
•Fast isolation—intact virus or EV’s in <15 minutes
•Simple automation—96 samples per run with KingFisher Purification System
•Functional virus—the intact virus can be used in any functional assay
•Release—the virus can be released from the beads in ∼10 minutesDownstream applicationsThe enriched intact virus can be used for further virus growth or in any downstream functional assays, protein research (western, mass spec), purification of RNA for PCR, etc. In addition, virus-like particles (VLPs), containing only virus-specific proteins and not nucleic acids, are extensively used in viral proteomic research. Dynabeads Intact Virus Enrichment beads isolate both live and inactivated intact viruses and VLPs.
Dynabeads Intact Virus Enrichment beads also isolate EV’s and exosomes and can, in addition to the applications above, be detected by flow cytometry using the Dynabeads-basedExosome-Human CD81 Flow Detection Reagent, for example, or the equivalent Dynabeads-based products forCD9orCD63. For analysis of other targets, the Dynabeads-basedExosome-Streptavidin Isolation/Detection Reagentcan be used in combination with biotinylated detection antibodies.
Easy and efficient protocolAfter a quick wash of the beads prior to use, the Dynabeads Intact Virus Enrichment beads are added to the sample in a short 10-minute incubation step. Wash the bead-bound virus once on aDynaMag magnetto remove the unbound target and add an appropriate buffer for 10 minutes according to the manual (below) if release from the beads is required. This quick and efficient isolation of viruses and EV’s is an improvement from the traditional and tedious ultracentrifugation methods that also require large and expensive equipment and give questionable yield.
Automation ready with the KingFisher Purification SystemThe Dynabeads magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of ourKingFisher sample purification systemsor liquid handlers.
Commercial supplyOur manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Intact Virus Enrichment beads in a commercial product or service or need a larger bulk volume of this product, please contact us atoemdynal@lifetech.
comor read more on ourDynabeads OEM page.
Certifications/Compliance: ISO9001 and ISO13485
Concentration: 40 mg/mL
Description: Strong anion exchange magnetic beads
Diameter (Metric): 1 μm
For Use With (Application): Virus Enrichment
For Use With (Equipment): KingFisher Sample Purification Systems, DynaMag Magnets
High-throughput Compatibility: High-throughput Compatible
Material: Magnetic Polystyrene
Quantity: 500 reactions
Regulatory Status: For Research Use Only
Shelf Life: 24 months from date of manufacture
Shipping Condition: Ambient Temperature
Surface Functionality: Strong positively charged surface
Target: Negatively charged viruses, exosomes, proteins
Uniformity: Monosized 1 μm (CV <5%)
Type: Dynabeads Intact Virus Enrichment
Unit Size: Each