Thermo Fisher, 11205D, Dynabeads™ M-280 Streptavidin

2 mL Dynabeads M-280 Streptavidin beads are 2. 8-μm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin. They are blocked with BSA and intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands. The excellent dispersion abilities and the absence of magnetic remanence make the Dynabeads streptavidin products ideal for both manual and automated protocols, including microfluidic system protocols. Benefits of Dynabeads M-280 Streptavidin beads include:
•Performance—high sensitivity and reproducibility secure consistent results
•Efficiency—fast binding kinetics and high binding capacity
•Flexibility—flexible protocols with gentle and efficient liquid-phase reaction kinetics
•Automation ready—easy automation for high-throughput needs on the Kingfisher Purification SystemConsistency and reproducibilityThe monolayer of streptavidin leaves most biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results. High binding capacityThe size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads M-280 Streptavidin typically binds:
• 490-750 pmol free biotin
• 10 μg biotinylated IgG
• ∼200 pmol biotinylated peptides
• ∼10 μg biotinylated ds-DNA
• ∼200 pmol biotinylated ss-oligonucleotidesApplicationsThermo Fisher streptavidin-coupled Dynabeads magnetic beads have been used and cited for a wide variety of applications. Although the beads can be used to isolate any biotinylated targets, these hydrophobic beads are particularly well suited for immunoassays, protein purification, cell isolation, phage display, and biopanning. Learn more about our streptavidin productsAutomation ready with the KingFisher Purification SystemThe 2. 8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of ourKingFisher Sample Purification Systemsor liquid handlers. Commercial supplyOur manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads M-280 Streptavidin beads in a commercial product or service or need a larger bulk volume of this product, please contact us atoemdynal@lifetech. comor see ourDynabeads OEM page. Bead Type: Magnetic Polystyrene
Binding Property: 650-900 pmoles/mg beads
Certifications/Compliance: ISO9001 and ISO13485
Color: Brown
Concentration: 10 mg/mL
Diameter (Metric): 2.8 μm
For Use With (Application): Capture of Biotinylated Targets
For Use With (Equipment): KingFisher™ Sample Purification System, DynaMag™ magnets
High-throughput Compatibility: High-throughput Compatible
Ligand Type: Streptavidin
Product Line: Dynabeads™
Product Type: Streptavidin Magnetic Beads
Quantity: 2 mL
Regulatory Status: For Research Use Only
Sample Type: Any Sample Type
Shelf Life: 36 months from date of manufacture
Shipping Condition: Ambient Temperature
Surface Functionality: Tosylactivated, Hydrophobic
Uniformity: Monosized 2.8 μm (CV <5%)
Format: Beads in suspension
Isolation Technology: Magnetic Beads
Unit Size: Each