Thermo Fisher, 11308D, Dynabeads™ Human DC Enrichment Kit

Dynabeads™ Human DC Enrichment Kit Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.
• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.
Methodology:An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.
Starting samples:PBMCCell Type: Dendritic Cells
Isolation Technology: Negative Isolation
No. of Cells: Processes ∼2 x 109cells total
Output Viability: >95%
Product Line: DYNAL™, Dynabeads™
Purity or Quality Grade: Research Grade
Quantity: 2 x 10 mL
Sample Type: PBMC
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 107PBMCs per isolation
Target Species: Human
Product Type: Cell Enrichment Kit
Unit Size: Each