Thermo Fisher, 11344D, Dynabeads™ Untouched™ Human T Cells Kit

Dynabeads™ Untouched™ Human T Cells Kit Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns requiredMethodology:An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommendedStarting Sample:PBMCCell Type: T Cells (Whole Population)
Final Product Type: Cells
Isolation Technology: Negative Isolation
No. of Cells: Processes ∼1 x 109cells total
No. of Tests: 100 Isolations
Output Viability: >95%
Product Line: DYNAL™, Dynabeads™, Untouched™
Quantity: 1 kit
Sample Type: PBMC
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 107PBMCs per isolation
Target Species: Human
Product Type: Cell Isolation Kit
Unit Size: Each