Thermo Fisher, 11346D, Dynabeads™ Untouched™ Human CD4 T Cells Kit

10 mL Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns requiredMethodology:An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.
Note:A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommendedStarting Sample:PBMCCell Type: T Cells (CD4)
Clonality: Monoclonal
Final Product Type: Cells
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Isolation Technology: Negative Isolation
No. of Cells: Processes ∼1 x 109cells total
No. of Tests: 100 Isolations
Output Viability: >95%, >98%
Product Line: DYNAL™, Dynabeads™, Untouched™
Purity or Quality Grade: Research Grade
Quantity: 10 mL
Sample Type: PBMC
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 107PBMCs per isolation
Target Species: Human
Volume (Metric): 10 mL
Product Type: Cell Isolation Kit
Unit Size: Each