Thermo Fisher, 11380037, MEM, suspension, no calcium, no glutamine

Quantity: 500 mL Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM is modified as follows:WithWithout
• Earle's salts
• L-glutamine
• Phenol Red
• HEPES
• CalciumThe completeformulationis available.
MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. This MEM formulation is made without calcium for use with suspension cultures. This MEM formulation contains Earle’s salts for use in a CO2incubator. This product is made with Earle’s salts.
Product Intended UseForin vitrodiagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type: Primary Rat Astrocytes
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: MEM (Minimum Essential Medium)
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Low Glucose, Phenol Red, Earle's Balanced Salt Solution (EBSS)
Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate, No Calcium
Unit Size: Each