Product Description
Grace's Insect Medium, unsupplemented Gibco™ Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth,Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth ofSpodeptera frugiperdacells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Gibco™ Grace's Unsupplemented Insect Medium can be used during transfection of insect cells, or for protein labeling studies (with methionine). We offer a variety of Gibco™ Media options for a range ofinsect cell culture applications.
This Grace's Insect Medium is modified as follows:WithWithout
• L-glutamine
• Lactalbumin hydrolysate
• Sodium bicarbonate
• Yeastolate
• MethionineThe completeformulationis available. Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate or yeastolate, commonly found in the supplemented version. Gibco™ Grace's Unsupplemented Insect Medium is also made without methionine for protein labeling studies.
Product UseFor Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
cGMP Manufacturing and Quality SystemGibco™ Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Grace's Unsupplemented Insect Medium requires supplementation with 10%Fetal Bovine Serum(FBS), 50 mg/L methionine, 3.
33 g/L lactalbumin hydrolysate and 3.
33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO2in order to maintain physiological pH.
Cell Line: Sf21, Sf9
Cell Type: Insect Cell
Inorganic Salts: Calcium, Magnesium
Product Line: Gibco™
Product Type: Grace's Insect Medium
Quantity: 500 mL
Shipping Condition: Room Temperature
Form: Liquid
With Additives: Glutamine, Sodium Bicarbonate
Without Additives: No Lactalbumin Hydrolysate, No Yeastolate, No Methionine
Unit Size: Each
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Collaboration
Tony Tang
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