Thermo Fisher, 11710035, Glasgow's MEM (GMEM)

Glasgow's MEM (GMEM) Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow's MEM was developed for use with kidney cell lines, such as BHK-21.
This Glasgow's MEM is modified as follows:WithWithout
• L-glutamine
• HEPES
• Phenol Red
• Tryptose phosphate brothThe completeformulationis available.
Using Glasgow's MEMGlasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.
75 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: BHK-21
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: MEM (Minimum Essential Medium)
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Sterility: Sterile-filtered
With Additives: High Glucose, Glutamine, Phenol Red
Without Additives: No HEPES, No Sodium Pyruvate, No Tryptose Phosphate Broth
Unit Size: Each