Thermo Fisher, 11753100, CellsDirect™ One-Step qRT-PCR Kit

100 reactions The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features of the CellsDirect™ One-Step qRT-PCR Kit include:
•Efficiency—offers significant time and cost savings for increased throughput
•Convenience—enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™TaqDNA Polymerase
•Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss
•Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells
•Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline your workflowIn traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for your qRT-PCR applicationCellsDirect™ technology is validated for gene expression studies involving limited samples. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (see figure). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.
g., TaqMan™ or LUX™ fluorogenic primers).
Note:This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-500) with ROX™ Reference Dye included in the 2X Reaction Mix is also available.
Concentration: 2X
For Use With (Equipment): 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
No. of Reactions: 100 reactions
Product Line: CellsDirect™, Platinum™, SuperScript™
Quantity: 100 reactions
Sample Type: Cells
Detection Method: Primer-probe
For Use With (Application): RT-PCR
Reaction Speed: Fast or Standard
Unit Size: Each