Thermo Fisher, 11754050, SuperScript™ VILO™ cDNA Synthesis Kit

50 Reactions The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.
The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.
Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, trySuperScript IV VILO Master MixorSuperScript IV VILO Master Mix with ezDNase Enzyme.
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.
Final Product Type: First-Strand cDNA
Format: Kit
No. of Reactions: 50 Reactions
Optimal Reaction Temperature: 42°C
Quantity: 50 reactions
Reaction Format: Master Mix
Reagent Type: Reverse Transcription
Reverse Transcriptase: SuperScript III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Size (Final Product): Up to 10 kb
Starting Material: RNA
Technique: Reverse Transcription
Detection Method: qPCR
For Use With (Application): Real Time PCR (qPCR)
GC-Rich PCR Performance: High
Reaction Speed: 60 min.
Unit Size: Each