Thermo Fisher, 12100046, DMEM, powder, high glucose

DMEM, powder, high glucose Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This DMEM is modified as follows:WithWithout
• High Glucose
• Sodium Pyruvate
• L-glutamine
• HEPES
• Phenol Red
• Sodium BicarbonateThe completeformulationis available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). DMEM uses a sodium bicarbonate buffer system (3.
7 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Line: HeLa, 293, Cos-7, and PC-12
Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: DMEM (Dulbecco's Modified Eagle Medium)
Quantity: 10 x 1 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: High Glucose, Glutamine, Phenol Red
Without Additives: No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
Unit Size: Each