Thermo Fisher, 12331013, One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells One Shot MAX Efficiency DH10B T1 Phage-ResistantE. colihave all the benefits of the DH10B genotype, with the addition of thetonA (also known asfhuA) genotype for resistance to T1 and T5 phage infection. The inclusion of thetonA genotype offers added security to protect valuable clones and libraries. MAX Efficiency DH10B T1 cells can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid, making them ideal for generating cDNA or genomic libraries, transforming large plasmids (>150 kb in size), and for situations when DNA is limiting.
MAX Efficiency DH10B T1 Phage-Resistant Cells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr), allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has thelacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, theendA1 mutation makes DH10B-T1R cells an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
MAX Efficiency DH10B T1 Phage-Resistant Cells offer:
• Transformation efficiencies of >1 x 109cfu/μg
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
•lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
•mcrA genotypic marker and themcrBC,mrrdeletion that allows for cloning DNA that contains methylcytosine and methyladenine
•endA1 mutation that enables increased plasmid yield and quantityConvenient and efficient formatDH10B-T1RE. coliare supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles, or money wasted on unused cells.
GenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74recA1endA1araD139 Δ(ara,leu)7697galUgalK λ-rpsLnupGtonAFind the strain and format that fit your needsWe offer other DH strains inchemically competentandelectrocompetent cellformats to meet your specific needs.
The DH10B-T1R strain is available inMultishot formatfor high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: High Copy Plasmid
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each