Thermo Fisher, 12574018, SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase The SuperScript III One-Step RT-PCR System with PlatinumTaqDNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and PlatinumTaqDNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.
5 kb. The amount of starting material can range from 0.
01 pg to 1 μg of total RNA.
Key advantages of SuperScript III One-Step RT-PCR System with PlatinumTaqDNA Polymerase
• Sensitive detection down to 0.
01 pg total RNA
• Convenient one-step format for increased speed, convenience, and less reaction-to-reaction variability
• SuperScript III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers
• Amplicon size—compatible detection of targets up to 4.
5 kb in length for greater flexibilitySuperScript III Reverse TranscriptaseSuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
PlatinumTaqDNA PolymerasePlatinumTaqDNA Polymerase is recombinantTaqDNA polymerase complexed with a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the denaturation step in PCR cycling at 94°C, providing an automatic 'hot start' in PCR for increased sensitivity, specificity, and yield.
Note:This kit has been optimized for end-point RT-PCR. For RT–qPCR in this product line, use the SuperScript III Platinum One–Step Quantitative RT-PCR System.
Final Product Type: PCR Amplified cDNA
Format: One-Step RT-PCR System
Hot Start: Built-In Hot Start
No. of Reactions: 25 Reactions
Optimal Reaction Temperature: 50°C
Polymerase: Platinum Taq DNA Polymerase
Quantity: 25 rxns
Reaction Format: Premixed Components
Reagent Type: Reverse Transcription
Reverse Transcriptase: SuperScript III
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
Size (Final Product): Up to 4.
5 kb
Starting Material: RNA
Technique: 1-Step RT-PCR
Detection Method: Gel Electrophoresis
GC-Rich PCR Performance: High
PCR Method: 1-step RT-PCR
Reaction Speed: 30 min.
Unit Size: Each