Thermo Fisher, 12634010, Advanced DMEM/F-12 Gibco™

Advanced DMEM/F-12 Gibco™ Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:With:Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol RedWithout:L-glutamine, HEPESThe completeformulationis available.
Using Advanced DMEM/F-12Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5%Fetal Bovine Serumand 4 mM L-glutamine orGlutaMAX™supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.
7 g/L) and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: MRC-5, SP2, Vero, WI-38 and Jurkat
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin
Form: Liquid
Serum Level: Reduced Serum
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
Without Additives: No Glutamine, No HEPES
Unit Size: Each