Thermo Fisher, 14013, Dynabeads™ M-450 Tosylactivated

Dynabeads™ M-450 Tosylactivated Dynabeads™ M-450 Tosylactivated are 4.
5 μm superparamagnetic beads containing surface tosyl groups. The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding. Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required.
• Quickly deplete cells or positively isolate target cells for downstream molecular analysis
• Excellent for cell isolation procedures using your own unique antibody
• Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activationCoupling procedureCovalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads. Optimal coupling occurs at high pH (8.
5–9.
5) and at 37°C. For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.
4.
Not recommended for immunoprecipitation (IP)These 4.
5 μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1 μm and 2.
8 μm Tosylactivated and Epoxy Dynabeads™.
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Cell Type: All cells from all species
Isolation Technology: Depletion, positive isolation for molecular applications
No. of Cells: Processes ∼2 x 109cells total
Output Viability: >95%
Product Line: DYNAL™, Dynabeads™
Purity or Quality Grade: Research Grade
Quantity: 5 mL
Reactivity: All species
Sample Type: PBMC, Tissue Digests, Blood
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 107cells per isolation
Target Species: All species
Diameter (Metric): 4.
5 μm
Product Type: Tosylactivated Bead
Unit Size: Each