Thermo Fisher, 15224041, T4 DNA Ligase (5 U/μL)

T4 DNA Ligase (5 U/μL) T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1). Single-stranded nucleic acids are not substrates for this enzyme. A T4 DNA Ligase Technical Bulletin is available.
Applications:Cloning (blunt-end or cohesive-end ligation) (2). Adding linkers or adapters to blunt-ended DNA (2).
Source:Purified fromE. coliœ lysogen NM989.
Performance and Quality Testing:Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency tested.
Unit Definition:One unit catalyzes the exchange of 1 nmol32P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)Unit Reaction Conditions:66 mM Tris-HCl (pH 7.
6), 6.
6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.
3 μM 32 P-labeled pyrophosphate, and enzyme in 0.
1 ml for 20 min. at 37°C.
Compatible Buffer: 5X Reaction Buffer
Product Type: T4 DNA Ligase
Quantity: 250 U
Shipping Condition: Dry Ice
Concentration: 5 U/μL
Enzyme: Ligase
Unit Size: Each