Thermo Fisher, 18010017, DNA Polymerase I

DNA Polymerase I DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.
•Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
•Source—purified fromE. coliexpressing the DNA Polymerase I gene on a plasmidPerformance and quality testingEndodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definitionOne unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions50 mM potassium phosphate (pH 7.
0), 6.
7 mM MgCl2, 1 mM 2-mercaptoethanol, 80 μg/mL template/primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μL for 30 min at 37°C.
Description: DNA Polymerase
Exonuclease Activity: 3' - 5', 5' - 3'
Hot Start: No
Polymerase: DNA Polymerase I
Quantity: 250 U
Shipping Condition: Wet Ice
Form: Liquid
Unit Size: Each