Thermo Fisher, 18021014, Ribonuclease H Invitrogen™

Ribonuclease H Invitrogen™ Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
ApplicationsRemoval of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
SourcePurified fromE. coliexpressing theE. coliRNase H gene on a plasmid.
Performance and quality testingRibonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.
Unit definitionOne unit hydrolyzes 1 nmol of RNA in3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.
Unit reaction conditions20 mM Tris-HCl (pH 7.
5), 0.
1 M KCl, 10 mM MgCl2, 0.
1 mM DTT, 5% (w/v) sucrose, 0.
5 nmol3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
Product Type: Ribonuclease H
Quantity: 30 U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Concentration: 2 U/μL
Enzyme: RNase
Unit Size: Each