Thermo Fisher, 18033100, T7 RNA Polymerase

2 x 2500 U T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.
Application:Synthesis of labeled and unlabeled RNA transcripts (1).
Source:Purified fromE. coliexpressing the T7 RNA Polymerase gene on a plasmid.
Performance and Quality Testing:3´ and 5´ exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in atranscription reaction.
Unit Definition:One unit hydrolyzes 1 nmol of ribonucleotide into acid-precipitable material in 1 h at 37°C using a T7 transcriptionvector as template.
Unit Reaction Conditions:40 mM Tris-HCl (pH 8.
0), 25 mM NaCl, 8 mM MgCl2, 2 mM spermidine-(HCl)3, 5 mM DTT, 0.
4 mM ATP, 0.
4 mM CTP, 0.
4 mM GTP, 0.
4 mM UTP, 1 μCi [3H]GTP, 1 μgSphI-cut pT7L13, and enzyme in 50 μl for 10 min. at 37°C.
Quantity: 2 x 2500 U
Polymerase: T7 RNA Polymerase
Promoter: T7
Unit Size: Each