Thermo Fisher, 18045088, CO2 Independent Medium

CO2 Independent Medium CO2Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2incubator. CO2Independent Medium is ideal for transporting cells or tissue and for the handling of mouse embryos under atmospheric conditions.
This CO2Independent Medium is manufactured as follows:WithWithout
• Phenol Red
• L-glutamine
• Sodium pyruvate
• HEPESThe complete formulation is confidential. For more information, please contactTechnical Support.
Gibco™ CO2Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate. A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions. No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems. Additionally, Gibco™ CO2Independent Medium has been formulated with components that enhance cellular production and utilization of CO2such that an exogenous source of CO2is not required for the maintenance of CO2dependent cellular functions.
Product UseFor research use only: Not intended for human or animal diagnostic or therapeutic uses.
Dual-Site cGMP ManufacturingGibco™ CO2Independent Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ CO2Independent Medium product made in our Scotland facility (18045-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
CO2Independent Medium contains no lipids or growth factors. Therefore, CO2Independent Medium requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). The CO2Independent Medium does not require a CO2environment to maintain physiological pH. For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2Independent Medium (seeprotocol).
Cell Line: Epithelial, fibroblast, and lymphoid
Concentration: 1 X
Green Features: Sustainable packaging
Product Type: CO2Independent Medium
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Species: Mouse Embryos
Form: Liquid
Sterility: Sterile-filtered
With Additives: Phenol Red, Sodium Pyruvate
Without Additives: No Glutamine, No HEPES
Unit Size: Each