Thermo Fisher, 21010046, Basal Medium Eagle (BME)

Basal Medium Eagle (BME) Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growthin vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is manufactured as follows:With:Phenol RedWithout:L-glutamine, HEPESThe complete formulation is available.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2incubator.
BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Line: HeLa, mouse fibroblasts, WI-38 and MRC-5
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: Basal Medium Eagle (BME)
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Sterility: Sterile-filtered
With Additives: Low Glucose, Phenol Red
Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate
Unit Size: Each