Thermo Fisher, 21127030, Ham's F-12K (Kaighn's) Medium

Ham's F-12K (Kaighn's) Medium Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
This F-12K (Kaighn's) Medium is manufactured as follows:With:L-glutamine, Phenol RedWithout:HEPESThe completeformulationis available.
Using Ham's F-12KHam's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors andFetal Bovine Serum(FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.
5 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Type: Human Hepatocytes
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: Ham's F-12K (Kaighn's) Medium
Quantity: 10 x 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives: No HEPES
Unit Size: Each