Thermo Fisher, 21700075, Ham's F-12 Nutrient Mix, powder

Ham's F-12 Nutrient Mix, powder Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This Ham's F-12 is modified as follows:WithWithout
• L-glutamine
• Sodium Bicarbonate
• Phenol RedThe completeformulationis available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). F-12 uses a sodium bicarbonate buffer system (1.
176 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Type: Primary Rat Astrocytes
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: Ham's F-12 Nutrient Mixture
Quantity: 10 x 1 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Sterility: Sterile-filtered
With Additives: Glutamine, Phenol Red, Sodium Pyruvate
Without Additives: No HEPES, No Sodium Bicarbonate
Unit Size: Each