Thermo Fisher, 22349, SIA (succinimidyl iodoacetate)

SIA (succinimidyl iodoacetate) Thermo Scientific Pierce SIA is a very short (1.
5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIA:
•Reactive groups:NHS esterandiodoacetyl
•Reactive toward:amino and sulfhydryl groups
• Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare toSIAB
• NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
• Iodoacetyl group reacts with sulfhydryl groups at pH > 7.
5 to form stable thioether bond
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)Product References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: Yes
Description: SIA
Form: Solid
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 283.
02
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Haloacetyl, NHS Ester
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 1.
5 Å
Water Soluble: No
Chemical Reactivity: Amine-Sulfhydryl
Cleavable: No
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Short (<10 Å)
Unit Size: Each