Thermo Fisher, 22551022, William's E Medium, no glutamine

William's E Medium, no glutamine William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:WithWithout
• Phenol Red
• L-glutamine
• HEPESThe completeformulationis available.
Gibco™ William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.
Dual-Site cGMP ManufacturingGibco™ William's E Medium is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ William's E Medium product made in our Grand Island facility (12551-032). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10%Fetal Bovine Serum(FBS). William's E Medium uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Type: Mammalian Cells
Product Line: Gibco™
Product Type: William's E Medium
Quantity: 500 mL
Shelf Life: 12 Months
Shipping Condition: Room Temperature
Culture Type: Mammalian Cell Culture
Form: Liquid
With Additives: Phenol Red, Sodium Pyruvate
Without Additives: No Glutamine, No HEPES
Unit Size: Each