Thermo Fisher, 23400021, RPMI 1640 Medium, powder, HEPES

RPMI 1640 Medium, powder, HEPES Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This RPMI is modified as follows:WithWithout
• L-glutamine
• Sodium Bicarbonate
• Phenol Red
• HEPESThe completeformulationis available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Intended UseForin vitrodiagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type IIDrug Master File(DMF).
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.
0 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type: Leukemic Cells
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity: 10 x 1 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Glutamine, HEPES, Phenol Red
Without Additives: No Sodium Pyruvate, No Sodium Bicarbonate
Unit Size: Each