Thermo Fisher, 26637, PageRuler™ Unstained High Range Protein Ladder

PageRuler™ Unstained High Range Protein Ladder Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
Features of PageRuler Unstained High Range Protein Ladder:Size range—8 proteins spanning 60 to 250kDaReady-to-use—supplied in a loading buffer for direct loading on gelsSharp bands—excellent accuracy for detection in with coomassie or silver stainsReference band—150kDa band has greater intensity for easy orientationTagged—each protein in the ladder contains an integral Strep-tag™ II SequenceQuality tested—each lot evaluated by SDS-PAGE and Western blottingThe protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
Includes:Proteins (0.
02 to 0.
05 mg/mL of each) in 62.
5 mM Tris-H3PO4 (pH 7.
5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.
01% bromophenol blue and 33% glycerolApplications:Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
Molecular Weight (g/mol): 250, 200, 150, 120, 100, 85, 70, 60 kDa
Product Line: PageRuler™
Product Type: Protein Ladder
Quantity: 2 x 250 μL
Ready to Load: Yes
Recommended Applications: Tris-acetate gels
Shipping Condition: Approved for shipment on Wet or Dry Ice
Stain Type: Unstained
System Type: Western Blotting, SDS-PAGE
Number of Markers: 8
Size Range: 60 to 250 kDa
Unit Size: Each