Thermo Fisher, 28025021, M-MLV Reverse Transcriptase (200 U/μL)

1,000 Reactions M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)25as template-primer.
Features of M-MLV Reverse Transcriptase include:
• Excellent thermostability with optimal activity at 37°C
• Can be used to synthesize first-strand cDNA up to 7 kb
• Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCRSourcePurified fromE. coliexpressing thepolgene of M-MLV on a plasmidPerformance and quality testingSDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA productUnit reaction conditions50 mM Tris-HCl (pH 8.
3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.
5 mM [3H]dTTP, 0.
1 mM poly(A), 0.
1 mM oligo(dT)25, 0.
1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°CConcentration: 200 U/μL
Final Product Type: First-Strand cDNA
Format: Stand-alone Enzyme
No. of Reactions: 1,000 Reactions
Optimal Reaction Temperature: 37°C
Quantity: 200,000 units
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Reverse Transcriptase: M-MLV
Ribonuclease H Activity: Yes
Shipping Condition: Wet Ice
Size (Final Product): Up to 7 kb
Starting Material: RNA
Technique: Reverse Transcription
Reaction Speed: 50 min.
Unit Size: Each