Thermo Fisher, 31800089, RPMI 1640 Medium, powder

RPMI 1640 Medium, powder RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This RPMI is modified as follows:WithWithout
• L-glutamine
• HEPES
• Phenol Red
• Sodium BicarbonateThe completeformulationis available.
Using RPMIRPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10%Fetal Bovine Serum(FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.
0 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type: Leukemic Cells
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity: 10 L
Shelf Life: 36 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Powder
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Glutamine
Without Additives: No Sodium Pyruvate
Unit Size: Each