Thermo Fisher, 31985070, Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced Serum Medium Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction ofFetal Bovine Serumsupplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ Imodificationsfor a range of cell culture applications.
This Opti-MEM™ I is modified as follows:With
• L-glutamine
• Phenol RedThe complete formulation is confidential. For more information, please contactTechnical Services.
Using Opti-MEM™ I MediumOpti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.
4 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: Sp2, AE-1, CHO, BHK-21, and HEK
Cell Type: Primary Fibroblasts
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™, Opti-MEM™
Product Type: Opti-MEM I
Quantity: 500 mL
Shelf Life: 18 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Human Origin
Form: Liquid
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: Glutamine, Phenol Red
Unit Size: Each