Thermo Fisher, 32571036, MEM α, nucleosides, GlutaMAX™ Supplement

MEM α, nucleosides, GlutaMAX™ Supplement Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM α is modified as follows:With
• Ribonucleosides
• Deoxyribonucleosides
• GlutaMAX™
• Phenol RedThe completeformulationis available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco™ MEM α withGlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM α uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type: Primary Rat Astrocytes
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™, GlutaMAX™
Product Type: MEM α (Minimum Essential Medium α)
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: Low Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without Additives: No HEPES
Unit Size: Each