Thermo Fisher, 4370074, TaqMan™ Gene Expression Master Mix

1 x 50 mL Get precise and reliable real-time qPCR quantification over nine logs of linear dynamic range with TaqMan Gene Expression Master Mix. TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target. Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend. TaqMan Gene Expression Master Mix has been validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications, including:
• Rare transcript detection
• Gene expression analysis
• Pathogen detection and viral load quantification
• Co-amplification of two targets
• Specific detection of gene family membersTaqMan Gene Expression Master Mix contains AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments.
Alternative product:TryTaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Gene Expression Master Mix and added additional capabilities for your gene expression analysis.
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
No. of Reactions: 2000 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Product Line: TaqMan™
Purity or Quality Grade: UP (Ultra Pure)
Quantity: 1 x 50 mL
Sample Type: DNA (Genomic), cDNA
Shipping Condition: Wet Ice
Volume: 1 x 50 mL
Detection Method: Primer-probe
For Use With (Application): Gene Expression
Form: Liquid
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each