Thermo Fisher, 4371353, TaqMan™ Genotyping Master Mix

TaqMan™ Genotyping Master Mix **********************Alternative Product:TryTaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation experiments.**********************TaqMan™ Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.
• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan™ SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysisMay be used for a variety of genotyping applications, including:
• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applicationsReaction size is based on a 50 μl reaction volume; please see below for other reaction sizes.
Exceptional Performance from an Optimized Mix (2X)Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan™ Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:
• AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems™ real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan™ AssaysValidated with TaqMan™ Genotyping Assays and InstrumentsTaqMan™ Genotyping Master Mix has been tested across all types of TaqMan™ SNP Genotyping Assays: TaqMan™ Drug Metabolism Genotyping Assays, TaqMan™ SNP Genotyping Assays, and Custom TaqMan™ SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems™ thermal cyclers and real-time PCR systems.
High-Throughput Setup and AnalysisThe combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan™ Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.
Reliable Discrimination with Challenging TargetsAmplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan™ Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.
Setting the Industry Standard for QualityExtensive analytical and functional tests are performed for each manufactured lot of TaqMan™ Genotyping Master Mix, including one TaqMan™ SNP Genotyping Assay and one TaqMan™ Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems™ Web site (search for 'Certificate of Analysis').
Number of Genotyping Reactions at a Given Reaction Size:4371355, TaqMan™ Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 μL Reactions: 400
• 20 μL Reactions: 1000
• 10 μL Reactions: 2,000
• 5 μL Reactions: 4,0004381656, TaqMan™ Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 μL Reactions: 800
• 20 μL Reactions: 2,000
• 10 μL Reactions: 4,000
• 5 μL Reactions: 8,0004371357, TaqMan™ Genotyping Master Mix, (1 x 50 mL)
• 50 μL Reactions: 2000
• 20 μL Reactions: 5,000
• 10 μL Reactions: 10,000
• 5 μL Reactions: 20,0004381657, TaqMan™ Genotyping Master Mix, (2 x 50 mL)
• 50 μL Reactions: 4000
• 20 μL Reactions: 10,000
• 10 μL Reactions: 20,000
• 5 μL Reactions: 40,000Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
Concentration: 2X
Fidelity (vs. Taq): 2 X
For Use With (Equipment): Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Genotyping Target: Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Product Line: TaqMan™
Product Type: Genotyping Master Mix
Purity or Quality Grade: UP (Ultra Pure)
Quantity: 1 x 1 mL
Sample Type: DNA (Genomic)
Technique: SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Volume: 1 mL
Detection Method: Primer-probe
For Use With (Application): Genotyping
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each