Thermo Fisher, 4427013, TaqMan™ Pri-miRNA Assay

750 Reactions TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.
•Highly specific—quantitiate miRNA transcription from a single genomic locus
•Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
•A new perspective—measure miRNA expression at the gene levelTaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.
Design and selectionSince all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.
Pre-formulated assay(20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)Concentration: 20X
Fidelity (vs. Taq): 60 X
Format: Tube
Green Features: Sustainable packaging
No. of Reactions: 750 reactions
Product Line: TaqMan™
Product Type: Pri-miRNA Assay
Quantity: M (750 reactions), made to order
Shipping Condition: Room Temperature
Sufficient For: 750 Reactions
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Fast
Unit Size: Each