Thermo Fisher, 4473088, TaqMan™ miRNA ABC Purification Kit - Human Panel B

TaqMan™ miRNA ABC Purification Kit - Human Panel B Use the TaqMan™ miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.
• Simple and fast workflow using oligonucleotide-conjugated Dynal™ magnetic beads
• Small sample input: typical volumes range from 10 μL for blood samples to 50 μL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol™ reagent, or guanidine isothiocyanateSimple and Fast miRNA PurificationThe TaqMan™ miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in 75 minutes (see workflow, Figure 1).
Human Panel A and Human Panel BThe TaqMan™ miRNA ABC Purification Kit is offered in two configurations:Human Panel Aand Human Panel B. The beads in each kit are superparamagnetic Dynabeads™ covalently bound to a unique set of 377 anti-miRNA oligonucleotides for each panel that correspond to the assays contained in the TaqMan™ Array Human MicroRNA A and B cards. The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads. Complete lists of miRNAs isolated by the panels can be found in the TaqMan™ miRNA ABC Purification Kit protocol (below).
miRNA AnalysisThe miRNAs isolated using the TaqMan™ miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using eithersingle tube (individual) TaqMan™ microRNA assaysor theTaqMan™ Array Human microRNA A and B cards. Figure 2 gives examples of the data that can be obtained from miRNA isolated from various sample types using the TaqMan™ miRNA ABC Purification Kit.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Final Product Type: Micro RNA
Green Features: Fewer resources used and less waste, Less hazardous
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Isolation Technology: Magnetic Bead
Kit Type: miRNA ABC Purification Kit
Product Line: TaqMan™
Purification Target: miRNA
Purification Time: 75 min
Quantity: 40 preps
Sample Type: Any Tissue Type, Cell Cultures, Tissue (FFPE), Whole Blood, Tissue Sections, Saliva, Serum, Urine, Plasma, Cultured Cells
Species: Human
For Use With (Application): miRNA analysis
Unit Size: Each