Thermo Fisher, 4485713, TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited The Applied Biosystems™ TaqMan™ GAPDH Assay with JUN™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN™ dye-labeled QSY™ probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.
Benefits of this assay include:
• Eliminate assay design time by using pre-designed primers and pre-designed JUN™ dye-labeled TaqMan™ probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC™ dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactionsSave time in assay design, formulation, and testing by using the TaqMan™ GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.
In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.
The JUN™ dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN™ dye) due to an overlap of spectra. TaqMan™ Multiplex Master Mix with MUSTANG PURPLE™ dye is recommended.
Accession Number: NM_002046.
3
Amplicon Size: 118
Format: Tube
Gene Symbol: GAPDH
Green Features: Sustainable packaging
Internal Probe Modification: QSY™ Non-Fluorescent Quencher (3')
No. of Reactions: 250 Reactions
Product Line: TaqMan™
Product Type: Gapdh Assay
Quantity: 250 reactions
Species: Human
Sufficient For: 250 Reactions
Target: Glyceraldehyde-3-phosphate dehydrogenase
Detection Method: Primer-probe
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
Label or Dye: JUN
PCR Method: qPCR
Unit Size: Each