Thermo Fisher, 452640, TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells

10 Reactions TOPO™ TA Cloning™ Dual Promoter kits are for fast, efficient cloning and subsequent in vitro transcription. These kits include the pCR™II-TOPO™ TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO™ cloning is the most reliable method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants. Convenient features of this TOPO™ TA Cloning™ Dual Promoter kit include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, includingEcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection inE. coli
• Easy blue/white colony screening for selection of recombinants
• No competent cells included for flexibility and cost-savingsKit Options: TOPO™ TA Cloning™ Dual Promoter KitsTOPO™ TA Cloning™ Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:
• General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)
• High-efficiency cloning: TOP10 Electrocomp™ Cells (Cat. Nos. K4660-01, K4660-40)
• General cloning, bacteriophage T1 resistance: DH5α-T1R cells (Cat. Nos. K4620-01, K4620-40)
• Fast growth: Mach1™ -T1R chemically competentE. coli(Cat. No. K4610-20)
• Repressor/induction needs: TOP10F’ cells (Cat. Nos. K4650-01, K4650-40)
• Provide your own cells (Cat. No. 450640)Antibiotic Resistance Bacterial: Ampicillin (AmpR), Kanamycin (KanR)
Cloning Method: TOPO™-TA
For Use With (Application): Cloning and subsequent in vitro transcription
Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water
Product Line: TOPO™
Product Type: TA Cloning Kit
Promoter: T7, SP6
Quantity: 10 Reactions
Shelf Life: All reagents are stable for 6 months when properly stored.
Vector: TOPO-TA Vectors
Format: Kit
Unit Size: Each