Thermo Fisher, 51300044, Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)

Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) ITS-A (Insulin-Transferrin-Selenium-Sodium Pyruvate) Supplement is used as a basal medium supplement in order to reduce the amount of Fetal Bovine Serum (FBS) used to culture cells. ITS-A is used for adherent cell cultures. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-A is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS. We also offer ITS-G and ITS-X supplements.
Using ITS-A SupplementInsulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenite is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media. Sodium Pyruvate is a precursor to amino acids, fatty acids, and cholesterol; it is an important intermediate in many biosynthetic pathways. The completeformulationis available.
cGMP manufacturing and quality systemITS-A Supplement is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Cell Type: Mammalian Cells
Concentration: 100 X
Culture Type: Mammalian Cell Culture
Quantity: 10 mL
Shelf Life: 18 Months
Shipping Condition: Room Temperature
Product Type: Transferrin
Sterility: Sterile-filtered
With Additives: Sodium Pyruvate
Without Additives: No Phenol Red
Unit Size: Each