Thermo Fisher, 60101, Dynabeads™ kilobaseBINDER™ Kit

Thermo Fisher, 60101, Dynabeads™ kilobaseBINDER™ Kit The Dynabeads kilobaseBINDER Kit is designed for immobilizing linear, biotinylated, long double-stranded (ds) DNA molecules (>2 kb). It combines streptavidin-coupled Dynabeads and a unique binding solution containing a patented immobilization activator that significantly increases the binding capacity for linear, large dsDNA fragments. This product provides an advantage whenever immobilization of a large quantity of long, biotinylated dsDNA is required.
Benefits of Dynabeads kilobaseBINDER Kit include:
•Performance—high binding capacity for >2 kb dsDNA molecules
•Efficiency—fast kinetics allows the isolation of chromatin within minutes
•Consistency—high batch-to-batch reproducibility secures consistent results
•Automation ready—easy automation for high-throughput needs on the Kingfisher Purification SystemConsistency and reproducibilityThe monolayer of streptavidin leaves most of the biotin-binding sites available for the binding of free biotin and biotinylated ligands. The specific and defined surface allows for efficient capture, separation, and downstream handling. In addition, the low size distribution (<5%) of the monosized beads and the streptavidin monolayer ensure negligible leakage, providing batch consistency and reproducibility of results.
Binding capacityThe size of the ligand and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and ligand and between ligands. Due to steric hindrance, the binding efficiency is significantly reduced when the fragment size of nucleic acid exceeds 2 kb. The Dynabeads kilobaseBINDER Kit activates long, linear dsDNA, neutralizing steric hindrance and facilitating binding to the bead surface. With two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization, one mg of Dynabeads kilobaseBINDER beads typically binds:
• ∼70 pmoles of a 4 kb biotinylated DNA fragment
• ∼80 pmoles of a 10 kb fragment per mg beads
• >195 μg (6 pmol) biotinylated 50 kb DNA fragment per mg beadsThe binding solution increases the binding capacity of biotinylated 4 kb DNA fragments >10 times compared to not using the binding solution.
ApplicationsThis product is useful for applications in cell biology (e.
g. building chromatin beads that mimic chromosomes for the study of self-organization of microtubules into bipolar spindles, building synthetic nuclei for studying RNA export from the nucleusin vitro, reconstituting nuclear movement along microtubules and examination of the molecular basis of this movementin vitro).
The kit is also suitable for efficient target enrichment for long-read sequencing. We recommend extending the biotinylated probe by PCR after target hybridization to help ensure the resulting long DNA fragment is double-stranded.
Learn more about our streptavidin productsAutomation ready with the KingFisher Purification SystemThe 2.
8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of ourKingFisher Sample Purification Systemsor liquid handlers.
Commercial supplyOur manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads kilobaseBINDER Kit in a commercial product or service or need a larger bulk volume of this product, please contact us atoemdynal@lifetech.
comor see ourDynabeads OEM page.
Bead Type: Magnetic Polystyrene
Certifications/Compliance: ISO9001 and ISO13485
Color: Brown
Concentration: 10 mg/mL
Diameter (Metric): 2.
8 μm
For Use With (Application): DNA Extraction
For Use With (Equipment): KingFisher™ Sample Purification System, DynaMag™ magnets
High-throughput Compatibility: High-throughput Compatible
Ligand Type: Streptavidin
Product Line: Dynabeads™, kilobaseBINDER™
Product Type: Magnetic Beads
Quantity: 200 isolations
Regulatory Status: For Research Use Only
Sample Type: Any Sample Type
Shelf Life: 36 months from date of manufacture
Shipping Condition: Ambient Temperature
Surface Functionality: Tosylactivated, Hydrophobic
Uniformity: Monosized 2.
8 μm (CV <5%)
Format: Beads in suspension
Unit Size: Each