Thermo Fisher, 65-0858-39, eBioscience™ Fura-2 AM Dye

eBioscience™ Fura-2 AM Dye Fura-2 is a calcium imaging dye that binds to free Ca2+. Fura-2 AM is the cell-permeable acetoxymethyl (AM) ester form of Fura-2. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when the alteration of excitation wavelengths is more practical than the detection of multiple emission wavelengths. Upon binding Ca2+, the excitation spectrum of Fura-2 shifts to shorter wavelengths between 300 and 400 nm, while the peak emission remains steady around 510 nm. The Kdof Fura-2 is highly dependent on pH, temperature, ionic strength and viscosity of the cytosol, thus great care should be taken when non-standard conditions are used.
Molecular weight: 1001.
86Peak excitation: variable depending on the concentration of free Ca2+, between 300 and 400 nmPeak emission: 510 nmFura-2 AM should be reconstituted in high-quality, freshly opened DMSO. Once reconstituted, it should be protected from light and stored at -20°C. Avoid freeze-thawing.
Reported ApplicationMicroscopy, Cell LabelingDetection Method: Fluorescence
Quantity: 1 mg
Emission: 300 to 400/510
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Product Line: eBioscience
Product Type: Dye
Unit Size: Each