Thermo Fisher, 72400120, RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This RPMI is modified as follows:With
• GlutaMAX™
• Phenol Red
• HEPESThe completeformulationis available.
Using RPMI 1640 MediumRPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium withGlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.
0 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type: Leukemic Cells
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™, GlutaMAX™
Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity: 10 x 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: GlutaMAX, HEPES, Phenol Red
Without Additives: No Sodium Pyruvate
Unit Size: Each