Thermo Fisher, A10072, Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)

Thermo Fisher, A10072, Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate) Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.
Fluorogenic Phospholipase A Substrates Specifications:
• Label (Ex/Em): Red/Green BODIPY™ PC-A2 (∼488/530,590 nm) (can be used in dual emission fluorescence ratio detection)
• Specificity: PLA2
• Cleavage product:D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry.
Find More Probes for Lipid Metabolism and Fatty Acid AnalogsReviewFatty Acid Analogs and Phospholipids—Section 13.
2andProbes for Lipid Metabolism and Signaling—Section 17.
4in the Molecular Probes™ Handbook for more information on these products.
For Research Use. Not for human or animal therapeutic or diagnostic use.
Cell Permeability: Cell-Permeant
For Use With (Equipment): Flow Cytometer, Microplate Reader
Product Line: BODIPY™
Quantity: 100 μg
Shipping Condition: Room Temperature
Substrate: Phospholipase A2 substrate (ratiometric)
Detection Method: Fluorescence
Form: Solid
Substrate Properties: Lipid-Based Substrate
Target Enzyme: Phospholipase
Unit Size: Each